Background: The main objective of this study was to evaluate the prevalence of tick infestation in cattle and buffaloes in Ballari. The prevalence was studied in relation to seasonal diversity. Results: The tick infestation was higher in cattle (73.47%) when compared to that of buffaloes (64.10%). The infestation rate was dominated by R. sanguineus (7.26%) followed R.haemophysaloides (6.96), R.evertsi (6.65) and B.microplus (6.65). The infestation of Haemaphysalis Canestrini was least with (3.74%) followed by Hyalomma Spp (2.90 %). The results of the present work confirm that the following ticks are dominant in domestic cattle and buffalo in Ballari region. Conclusions: The present study revealed that the rate of tick infestation was higher in monsoon followed by summer and winter. The study has been able to document the common species of ticks infesting cattle and buffaloes. The epidemiological data can be used for strategic tick control programme and the study recommends that care should be taken in proper maintenance of the animals, sanitation and also awareness needs to be created among the cattle owners. Regular treatment to be provided to animals infected with ticks, proper and regular feeding techniques to be undertaken, upgradation of management practices to be done in order to reduce and prevent the ectoparasitic infestations in Ballari region, Karnataka.