The hypopharyngeal glands (HGs), the most important organ in Hymenoptera, are crucial for insect physiology. The royal jelly that is fed to queens and larvae is made possible by the (HGs) in honeybee worker bees. This study examined the effects of inorganic iron (Fe) addition on the development and structure of the (HGs). We tested the effects of high iron supplementation on Apis mellifera jemenatica hypopharyngeal gland growth during the off-season using various concentrations of inorganic iron. With three hives in each group, we randomly divided 12 beehives into the following four treatment groups: Fe0 (without any additional Fe), Fe25, Fe50, and Fe100 (each with an additional 25, 50, and 100 mg L−1 Fe). The treatment lasted three months. Each group's six-day-old nursing bees were sectioned, and the sections were then viewed under a microscope to study the morphology of the glands. A reliable measure of whether Fe supplementation levels surpassed the dietary requirements for iron that might alter HPG development supplied to nursing bees is the mass and size of the HGs. The formation of HPG may be impacted by iron, according to ANOVA results. (Apis mellifera L.) nutritional requirements were exceeded by Fe supplementation levels, which had a significant impact on HG mass and acini size. In the current investigation, we discovered the impact of various Fe supplementation levels on nursing honeybee workers' HGs development and acini size.