Biological diversity refers to the variety of life on Earth including plants, animal, Bacteria and Fungi. Butterflies are one of the most important assemblages of insects that act as bio indicators as well as nature’s gardeners. The present paper is based on the Survey Report on the Butterfly fauna of Jabalpur Region, Madhya Pradesh. Faunistic surveys were undertaken during July 2021- August 2022 and a total of 1143 individuals Lepidoptera (Rhopalocera) belonging to 47 species, 39 genera under 04 families from various localities in and around the Jabalpur, of which the members of family Nymphalidae is outnumbering the other butterfly families. Moreover, the study revealed 08 butterfly species newly recorded in the Central Indian landscape. Shannon-Weiner diversity index of the study site is -3.506764433 showing high diversity which is inversely proportional to Simpson Index value that is 0.034935156. The area of study having high organic pollution and rich diversity of butterflies, therefore it should be of great importance for conservation. The aim of this research is to contribute to the development of planning and management strategies for diversity restoration in the studied area.