This investigation aims to understand the morphometric characters and DNA barcoding of Bactrocera fruit flies, which originated from citrus plantations in Simalungun, North Sumatra, Indonesia. The research goals also to comprehend the relation between fruit flies' body length and other morphometric features. Fly trapping was done in Baringin Raya village using the Steiner trap. Classic taxonomy and DNA barcoding methods have been used to identify fruit flies. The character of morphometric: The length of the body (Y), the length of the wing (X1), the width of the wing (X2), and the length of tibia (X3) were observed and measured under a microscope. DNA barcoding has been done by isolation, amplification, electrophoresis, sequencing, and blasting. MEGA XI software was used to blast the PCR product into the NCBI gene bank. The calculation of morphometric traits and their relations were determined using SPSS v. 23. The research finding revealed that the fruit flies collected from oranges plantation species were Bactrocera dorsalis complex. The body, wing, tibia length, and wing width of obtained fruit flies (n=30) were 6.91, 6.36, 1.98, and 2.43 mm, respectively. The equation of multiple regression that stated the relation among body length (n=30) into other morphometric features is Y = 0.24 X1+ 0.58 X2 + 3.91 (R2=73.20). Wing length and wing width are traits that have the highest value in determining a fruit fly's length body. The size of the nucleotide base chain of obtained B.dorsalis fruit fly was about 690 bp.