The spoiled fish (Yellow goatfish – Sulphureus cuvier) was collected from three different locations viz., Chidambaram fish market, Killai, and Bhuvanagiri. The collected spoiled fish samples were preserved in the refrigerator at 4 ºC. Five bacterial isolates were isolated and identified from spoiled yellow goat fish. The Spoilage Bacteria - I was identified as Escherichia coli, Spoilage Bacteria - II was identified as Vibrio cholera, Spoilage Bacteria - III was identified as Staphylococcus aureus, Spoilage Bacteria - IV was identified as Salmonella typhi and the Spoilage Bacteria - V was identified as Pseudomonas fluorescens. The bacterial flora of fish incensed for human consumption depends on the environmental conditions of its natural habitat. The genera present in the gut generally seem to be those from the environment or diet.