The purpose of this study is to investigate the anti-snake venom properties of indigenous herbs traditionally used in the western ghats of India. It was discovered that traditional healers in Maharashtra, India utilised these plants to treat patients bitten by snakes. The Daboia russelli and Naja naja freeze-dried venom powder was collected from the Hindustan Snake Park in Kolkata. A botanist recognised and confirmed the species Helicteres isora and Bixa orellana. Extraction of leaves from both plants using a hot extraction process, followed by evaluation for antivenom activity in vivo utilising a lethality assay as a research model.