Brinjal, Solanum melongena L. of the family, Solanaceae is grown twice to thrice a year. It can give a good handsome amount of produce. It has good nutritious value and even used in traditional medicine. Several biotic and abiotic factors destroyed the plants and fruits of brinjal, thus, reducing a great size in yield. The diseases which affect the yield and destroy the brinjal are Cercospora leaf spot, Colletotrichum fruit rot, Damping off, Early blight, Phomopsis fruit rot and Powdery mildew. And the major insect pests which destroy the brinjal yields and plants are Shoot and fruit borer, Whitefly, Leafhopper, Stem borer, Brinjal hadda beetle, Brinjal lace wing bug, Stem borer, Aphid Brown leaf hopper and Ash weevils. Brinjal shoot and fruit borer, Leucinodes orbonalis is the most harmful and destructive pest for brinjal. Many strategies have been put forward for the management of Brinjal fruit and shoot borer. The several control measures include the use of chemical or bio control agents or conventional physical method (removal of affected terminal shoot and fruits, avoiding continuous cropping of brinjal). Another method is growing the varieties with long and narrow fruits in endemic areas. Installation of pheromone traps. However, in current context the problem is insecticides resistant Fruit and shoot borer (Leucinodes orbonalis) are existed. So, it is very important to study and analyze the genetic variability among different fruit and shoot borer populations. Various molecular markers are deployed to study the genetic diversity of Leucinodes orbonalis. Many studies revealed that there is significant variation among the Leucinodes orbonalis populations collected from different locations.