Background: High fat diet induced obesity is the most challenging health problem which leads to other comorbidities worldwide. Obesity is a disorder either due to high calorie diet, circadian disruption or sedentary lifestyle. High fat diet (HFD) resulted in increased obesity and needs urgent solutions to overcome health issues. Objective: The present study is an attempt to understand the importance of time-restriction feeding (TRF) to reverse HFD-induced obesity. The current study aims to investigate the adverse effects of a high-fat diet on odd-time eating and high-fat diet with that of time-restriction feeding. Material Method: To address this issue, Drosophila melanogaster were chosen flies that were fed with different food regime such as high-fat diet (HFD), a normal diet (ND) as a control, HFD+TRF and ND+TRF. The assays were used to detect obesity, body weight, size and lipid profile. The locomotor, longevity and CAFÉ assay were performed to access the effect of TRF in obesity related activity. Conclusion: In results it was found that, TRF has an effective response to obesity by reducing body weight and adiposity caused by a high-fat diet. TRF diet also improves the muscle activity and overall body metabolic function, reflecting in the reduced body weight. These results indicate the efficacy of TRF regimen might be a potential novel non-pharmacological strategy to reverse the high fat diet induced obesity.