The present study aimed to investigate the aqueous extract of neems' bark and leaves against larvae mosquitoes in Khartoum State, Sudan. A. indica leaves and bark were collected from the Faculty of Public health and Environmental Health, University of Khartoum and identified and classify by Department of Botany Faculty of Science, University of Khartoum, the plant material washed by D.W water and alcohol then dried in shadow. Mosquito larvae (early 3rd and fourth instar) were collected from breeding sites pool of stagnant water from Gaba-alsunt-Khartoum state in a plastic bucket. Bioassay was applied according to WHO method in each test containing 20 leaves in 200 ml tap water, three replicates for each concentration of extract were done. after 24 hours the number of mortality was observed. The results were performed by calculating the death rate in each concentration The Porbit analysis was done using Excel 2016 to perform lethal concentrations for 50% of the mortality, LC50 and LC 95 at 24h after treatment.at different concentrations. the results obtained from A. indica leaves and blend (bark + leave) water extracts were applied to mosquito larvae at concentrations, of 0.0125, 0.01, 0.005, 0.0005 and 0.00025 mg. The leaf extract was shown a range of mean mortality of between 45% and 95% after 24 hours and between 45%_and 98% after 48 hours. While water Extract A.indica blend (bark + leave) showed a range of mean mortality of between 50% and 90% after 24 hours and between 40%_and 95% after 48 hours. The probit analysis showed that the LLC50swas 0.0004 mg, 0.00039mg,0.00025 mg, and 0.00038mg after 24 hours for leaf and blend, respectively, while the LC95s were 0.0125 mg, 0.012mg, 0.0158 mg and 0.0125mg after 48 hours for leaf and blend, respectively.