Aims: UTIs are a frequent health issue. females were more likely to be affected by human concerns than males. Rare Staphylococcus spp bacteria were identified and studied for virulence characteristics, such as the presence of hemolytic enzymes, by both phenotypic and genomic analysis. Methodology and results: Urine was collected from 170 patients (100 females and 70 males) at Mosul General Hospital and Ibn Sina Teaching Hospital in Mosul city between (1/12/2021) and (30/3/2022), where phenotypic and partial detection of its virulence factors associated with the disease were conducted. Women were more likely to contract a urinary tract infection than men were; although men accounted for 29.4% of those infected, women accounted for 70.5%, and more than half of the bacteria isolated from female patients were Gram-negative. The bacterial isolates were primarily of Gram-positive bacteria, with Escherichia coli (30.36 percent), Pseudomonas aeruginosa (7.14 percent), Kleipsella pneumoniae (6.25%), Proteus mirabilis (2.6 percent), and Cromobacterium violaceum (2.6 percent) among the most common. The percentage of staphylococci where the coagulation test result was negative was (1.7%) and was due to 44.1% for staphylococcus aureus. Staphylococcus haemolyticus as a percentage. staphylococcus, epidermis (8.93), (9.82), and (2.68 xylosus) percent. Important bacteria like E. faecalis, S. aureus, S. simulans, S. lugdunensis, Leuconostoc mesenteroides spp., and S. hominis were isolated from the rare bacteria that made up 0.89 percent of our study's total bacterial isolates Conclusion, significance and impact of study: Hemolysin's damaging effect on kidney cells contributes significantly to urinary tract disease. The knowledge that it is produced by the Staphylococcus genus is crucial to understanding its etiology. There are three subtypes of hemolysin, each corresponding to a different gene: HLA for alpha hemolysin, HLB for beta hemolysin, and HLD for dalta hemolysin. GeneKeywords: Genes, Molecular bacteriological, Staphylococcus spp, Urinary tract infections.