Janaki Forest is a known tourist place in Kozhikode District, Kerala. The area is protected by the Forest department of Kerala under the Kuttiyadi Forest range. The present study highlights the significant record of butterfly diversity of Janaki Forest. The study was conducted from January 2022 to April 2022 by applying transect count method. Butterfly species diversity was assessed quantitatively across the different habitats in the study area. The study site-1 represent riverine, the site-2 represent wet land, the site 3 represent evergreen forest and the site-4 represents mixed vegetation. The site-3 evergreen forest is observed as the area with maximum species diversity, followed by site-1 riverine and site-4 mixed vegetation. Site-2 wet land is observed as the area with less species diversity. The area known for riparian type of habitat that serves as a good host for various species of butterflies. During the study period, a total of 60 butterfly species belonging to 6 families were recorded with Nymphalidae as the dominant family with 24 species followed by Papilionoidea with 13 species, Pieridae with 11 species, Lycaenidae with 7 species, Hesperiidae with 4 species and Riodinidae with only one species. The difference in species diversity is because of differential distribution of various host plants, Availability of nectar resources and sunlight. Human activity also found to be a factor for the difference in diversity across various habitats.