A Study was carried out to identify 10 isolation of salmonella spp previously detected from beef and chicken meats by using pcr and real time pcr as well as 5 isolation were used for sequencening and specific genetics virulence gene type of salmonella spp . our results revealed that 10 isolation (100%) were identified on salmonella Spp by conventional Pcr . our finding also found out 5 isolation which were subjected to the detection of inva by using Rt -Pcr were reported salmonella enterica concerning sequencing and sequence analysis of salmonella 2 beef isolation (out of 2 examined) were reported as S. Agona, which 3 chicken isolates (out of 3 examined) were reported S. Infantis.