The vine eudemis (Lobesia botrana) is the main pest responsible for a considerable loss in the genus Vitis worldwide. The egg-laying behavior in the female is oriented on the fruiting organs on this basis our work is oriented in order to determine the preferences of laying. This study provides information necessary for egg-laying behavior in a potential pest of the vine, our objective is to determine the egg-laying preferences in this insect in order to master future blooms to develop relevant management measures for this insect. The study was approached by two different samples: destructive sampling and visual sampling. The results have made it possible to deduce that the number of eggs present on clusters varies significantly according to the years, the grape varieties studied and the different generations of the eudemis. The microdistribution of the eggs was studied according to the high or low position on the berries. Significant variability in the presence of eggs on clusters and the number of L. botrana eggs per cluster were observed between generations, years and grape varieties. Egg-laying preference varies according to grape varieties, generations and years.