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VOL. 6, ISSUE 6 (2021)
Larvicidal bioefficacy, antioxidant activity and cytotoxicity assessment of lemongrass oil nanoemulsion for controlling dengue vector, Aedes aegypti
Authors
Ami Verma, Shabad Preet
Abstract
Present study highlights the use of lemongrass essential oil in developing kinetically stable nanoemulsion for larvicidal application with major focus on addressing safety concerns. Various oil and surfactant ratios were evaluated for preparing stable oil in water nanoemulsion of lemongrass oil and 1:1 ratio exhibited great thermodynamic stability as recorded through dynamic light scattering study. Fourier-transform infrared spectroscopy (FTIR) analysis depicted the characteristic peaks at 1737.62 cm−1 and 1638.50 cm−1 of α, β- unsaturated esters (C-O), and conjugated alkene (C=C). Transmission electron microscopy (TEM) analysis revealed spherical droplets with smooth surface and the size was estimated as 38.1nm. Lemongrass nanoemulsion exhibited higher antioxidant activity as evident from the DPPH (85.17% at) and NO (72.4%) free radical scavenging activity. The enhanced larvicidal activity was observed at 24h, 48h and 72h of exposure against Aedes aegypti larvae and the LC50 value was calculated as 2.07ppm after 24h of exposure. Cytotoxicity assay against normal human renal cells revealed higher cell viabilities at lower concentrations after 24h and 48h did not show any apparent cytotoxicity impact at different concentrations. Current research validated the safer development from lemongrass essential oil with promising larvicidal application which may be further utilized as wider therapeutic agents.
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Pages:153-159
How to cite this article:
Ami Verma, Shabad Preet "Larvicidal bioefficacy, antioxidant activity and cytotoxicity assessment of lemongrass oil nanoemulsion for controlling dengue vector, <em>Aedes aegypti</em> ". International Journal of Entomology Research, Vol 6, Issue 6, 2021, Pages 153-159
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