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VOL. 6, ISSUE 6 (2021)
Biosynthesis of silver nanoparticles from Annona squamosa L. and evaluation of its insecticidal activity
Authors
M Gomathi, G Maheswari
Abstract
Nanotechnology is one of the most promising advanced approaches for pest control in recent years. The present investigation was carried out in the laboratory to evaluate the insecticidal activity of Annona AgNPs particles against the insect pest of green gram (Vigna radiata). Green biosynthesis of silver nanoparticles (AgNPs) was prepared using Annona squamosa leaf extract. The synthesized Annona AgNPs were characterized using UV-vis spectroscopy, FT-IR, TEM, and X-ray diffraction analysis. The phenolic compounds in the aqueous leaves extract pave the way for the possible reduction of silver to nano-silver. XRD- profile of biosynthesized AgNPs revealed that the average size of particle size was 22 ± 6 nm. UV-Vis spectral analysis of Annona AgNPs showed an absorption peak at 315nm. The spectral domine of colloidal solution with broader electronic adsorption bands reveals the occurrence of small spherical silver nanoparticles. FTIR Spectroscopic analysis revealed strong peaks at 3973.36, 3950.22, 3379.29, 2931.80, 2376.30, 2330.01, 1604.77, 1419.61, 1280.73, 107.42 and 624.94 cm-1. The strong bands at 624.94cm-1 are attributed to the stretching vibration of the C-CL functional group of alkyl halide. The peaks at 1072corresponds to C-N stretching vibration aliphatic amines, and weaker bands at 1280.73cm-1 represent the in-plane bending vibration of the C-N stretching vibration of aromatic amines. The absorption peaks at 1419.61 and 160.77cm-1 are assigned to the bending vibration of N-H amine. The smaller peak at 2931.80 and broader band at 3379.29 were identified as C-H stretching of alkanes and stretching vibration of N-H amine, respectively. TEM analysis shows that the Annona AgNPs particles were spherical metal particles with an average size of 162nm. The contact bioassay method was followed to assess the insecticidal activity of Annona AgNPs against the pulse beetle, C. analis. The results revealed that cent percent death was observed on VII day in 100% concentration and showed its effectiveness. The concentration of 60 and 80% were equally effective in arresting C. analis population in green gram. From the study, it was obvious that the Annona AgNPs particles were the most powerful, safe, non – toxic natural insecticides that can be used to manage bruchid infestation during storage.
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Pages:29-34
How to cite this article:
M Gomathi, G Maheswari "Biosynthesis of silver nanoparticles from <em>Annona squamosa</em> L. and evaluation of its insecticidal activity ". International Journal of Entomology Research, Vol 6, Issue 6, 2021, Pages 29-34
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