Incorporation of [3H]-cholesterol in various tissues during ovarian development of Locusta migratoria L. (Orthoptera: Acrididdae)

Versha Goel; Rita Rath; Ranjana Saxena

Incorporation of [³H]-cholesterol in various tissues during ovarian development of Locusta migratoria L. (Orthoptera: Acrididdae)

Authors
Versha Goel, Rita Rath, Ranjana Saxena

Abstract
Experiments were conducted to study the incorporation of [³H]-cholesterol in different tissues of adult female locust Locusta migratoria (5 days, 12 days and 22 days old). The results showed that radioactivity from the haemolymph was taken up by all the tissues namely fat body, ovary and muscles. The concentration of tritium in the haemolymph was initially more in all the three stages of development studied but declined gradually with time and cholesterol was incorporated in other tissues of the locust body. The fat body of the female locust picked up radioactivity more in younger stages i.e. 5 days (5.01%) and 12 days (6.86%) after the adult moult as compared to the fat body in 22 (3.38%) days old adult 8 hours after injection. The uptake of radioactivity by the immature ovary in 5 days (1.26%) old locust was very little which, however, increased a little in 12 days (3.03%) old females but tremendously in 22 days (19.21%) old adult 16 hours after injection. This may perhaps be correlated with development of oocytes which occurs between 12 and 22 days. The uptake of cholesterol was comparatively rapid in muscles of 12 days (9.89%) old adults 8 hours after injection.

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