Biofloc technology is regarded as an approach that produces microbial flocs by degrading organic waste by microorganisms. The present study was executed to evaluate the effects of partial substitute of every day feeding intake with biofloc, and the histology of Litopenaeus vannamei a prawn species. The species was collected from the culture ponds during the period of December to march from Thirunagari, Tamil Nadu, India and were brought to the laboratory in live condition using seed bags for carrying out histological examination. Histological appearance of Hepato-pancreas, anterior intestine and stomach were observed for both control and biofloc cultured prawn. The obtained results clearly indicated that there were no significant histological alterations found between the control and Biofloc technology shrimp farming prawn. Only little more differences were observed in all three organs of biofloc shrimp farming prawn compared to the prawn from normal shrimp farming and the reason for this is not clear. There were no pathological changes and neat arrangements of cells in these organs evidenced the key role in digestion, absorption and storage of food in L.vannamei.