Permanent alterations in the Drosophila fauna would suggest substantial biotic changes at least at the level of plants, fungi, and hymenopterous wasps that parasite upon various life cycle stages of Drosophila. Since the long year times, drosophila has been used in genetical, cytological and evolutionary studies. In this connection fruit flies are admirable experimental animal. Hence, in the current work we studied the relative abundance of Drosophila species in an around Amarkantak region, Madhya Pradesh from February to April in the year 2019. 15,533 numbers of flies were collected in total comprising of seven different species of Drosophila i.e. D. busckii, D. melanogaster, D. ananasae, D. Simulans, Zaprionus indianus, D. jambulina and D. immigrans. D. busckii showed highest relative abundance of 23.21 % while D. annanassae showed the least relative abundance of 10.64 %. This study is the first of its kind to study the relative abundance of Drosophila species in and around Amarkantak region, Madhya Pradesh during the spring season. We also accessed the diversity of Drosophila species with the application of Simpson (D’), Shannon (H’) and Berger-Parker (1/d) index showing the abundance, species richness and diversity of Drosophilids. Application of these indices to study different measures of Drosophila biodiversity in different months demonstrated that the month of March had a highest value of Simpson (D’), Shannon (H’) index suggesting the month has highest biodiversity of Drosophila compared to other months.