ARCHIVES
VOL. 6, ISSUE 5 (2021)
In silico and In vitro anti-mosquito analysis of some secondary metabolites from Citrus species on Aedes aegypti and Culex quinquefasciatus
Authors
T Jayapriya, A Elangovan, R G Manivel
Abstract
The plant-based insecticides are a reliable and eco-friendly alternative to synthetic chemicals. Therefore, the aim of the present investigation is to identify the best insecticidal activity of secondary metabolites from Citrus species peel, such as naringenin, naringin, hesperidin and diosmin, by in silico analysis. Further, the same was analyzed for larvicidal, pupicidal and adultcidal activities, and histopathological changes in the midgut of Aedes aegypti and Culex quinquefasciatus mosquitoes. Thus, based on the in silico analysis, naringenin showed the highest binding affinity towards the target protein AeSCP-2 with least binding energy value of -9. 75511 kcal/mol. Moreover, naringenin showed larvicidal and pupicidal activities after 24 h of exposure of naringenin at a different concentration like 2, 4, 6, 8 and 10 ppm. The larval mortality of LC50 and LC90 values of naringenin were 3. 537 and 9. 940 ppm against Ae. aegypti, meanwhile 3. 537 and 9. 940 ppm for Cu. quinquefasciatus. The pupal mortality at LC50 and LC90 were 3. 418 and 9. 848 ppm for Ae. aegypti; 3. 700 and 10. 009 ppm of naringenin for Cu. quinquefasciatus, respectively. The adutcidal effect was observed for naringenin at 3, 6, 9, 12 and 15 ppm against the mosquitoes. The adult mortality value of LC50 and LC90 were 5. 390 and 14. 701 for Ae. aegypti while 5. 719 and 15. 138 ppm for Cu. quinquefasciatus, respectively. Furthermore, histopathological examination also clearly shows destructive midgut regions in Ae. aegypti and Cu. quinquefasciatus. Thus, the present results demonstrated that naringenin is as good candidate for natural insecticide production that can help control the mosquito proliferation.
Download
Pages:96-104
How to cite this article:
T Jayapriya, A Elangovan, R G Manivel "<em>In silico</em> and <em>In vitro</em> anti-mosquito analysis of some secondary metabolites from <em>Citrus </em>species on <em>Aedes aegypti</em> and <em>Culex quinquefasciatus</em> ". International Journal of Entomology Research, Vol 6, Issue 5, 2021, Pages 96-104
Download Author Certificate
Please enter the email address corresponding to this article submission to download your certificate.