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VOL. 6, ISSUE 5 (2021)
Greater wax moth of Galleria mellonella (Lepidoptera: Pyralidae) as a model host for human pathogen in Escherichia coli
Authors
Aysha A, Chitra P
Abstract
Larvae of Galleria mellonella (greater wax moth or honeycomb moth) are a widely used model for studying virulence factors and pathogenesis of many prominent bacterial and fungal human pathogens, the efficacy of new antimicrobial compounds, and immune responses. G. mellonella larvae are inexpensive easily obtained and are simple to use as they don’t require special lab equipment. The immune system of G. mellonella larvae is structurally and functionally analogous to the innate immune response of mammals, which makes this model suitable for study of virulence and pathogenesis of human pathogens. The present research is undertaken to assess the suitability of G. mellonella as an alternative model for studying the virulence and pathogenicity of Human pathogen E. coli and also the cellular and humoral immunity of E. coli to G. mellonella. Mortality and Survival record of the larvae challenged with 10 μl of105 to107 CFU/Ml and with 0.1% peptone water as control were observed over 72 hrs and dose responses data obtained. Phenoloxidase enzyme activity was investigated as a marker for immune response. Results demonstrate that E. coli is pathogenic towards G. mellonella Infection with E. coli strains activated the melanisation pathway resulting in melanin deposition. The findings suggest G. mellonella might be a useful in vivo model of infection and convenient as a prescreening assay for virulence of E. coli.
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Pages:42-47
How to cite this article:
Aysha A, Chitra P "Greater wax moth of<em> Galleria mellonella </em>(Lepidoptera: Pyralidae)<em> </em>as a model host for human pathogen in <em>Escherichia coli</em> ". International Journal of Entomology Research, Vol 6, Issue 5, 2021, Pages 42-47
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