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VOL. 6, ISSUE 4 (2021)
Genetic polymorphism in the field populations of Pectinophora gossypiella (Saunders) by using RAPD-PCR
Authors
R V Kalaimathi, R Umamaheswari, S Krishnamoorthy
Abstract
The populations of Pectinophora gossypiella obtained from different locations in Tamil Nadu were analyzed by RAPD-PCR. A total of twenty 10-mer oligonucleiotide primers (RAPD Kit A1 to Kit A20) were tested for their ability to provide suitable banding pattern for eight different populations of P. gossypiella. Of the primer set tested, RAPD KitA3, A9, A15, A18 and A20 yielded clear, consistent and discrete banding patterns for the populations of P. gossypiella. Dendrogram and similarity index were constructed based on the RAPD profiles generated by Kit A3, A9 and A15 primer using UPGMA. The three primers amplified a total of 115 scorable bands in the molecular weight range of approximately 267bp to 1352bp. The number of amplification products obtained was specific to each primer and ranged from thirty (Kit A9) to forty five (Kit A15). The UPGMA based dendrogram of all the primers grouped the populations of P. gossypiella in two clusters. The similarity coefficients values were ranged from 0.03 to 0.86, indicating that no two populations were 100% similar. The similarity matrix of all the primers indicated that most of the populations exhibited <50% similarity coefficient.
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Pages:188-192
How to cite this article:
R V Kalaimathi, R Umamaheswari, S Krishnamoorthy "Genetic polymorphism in the field populations of <em>Pectinophora gossypiella</em> (Saunders) by using RAPD-PCR ". International Journal of Entomology Research, Vol 6, Issue 4, 2021, Pages 188-192
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