Dried fruit seeds; i.e. date pits powder of date palm trees (Phoenix dactylifera L.) swia variety, wild custard apple seeds of (Annona senegalensis P.) mandarin seeds, of (Citrus reticulate B.); considered as new bioactive compounds. They were used, as dust application, to control cotton leafworm, S.littorais (Boisd.).The biological studies and influence of this natural compounds on the growth and development of 2 nd and 4th instar larvae were investigated. The lethal time of larval mortality recorded after 24h, 48h, 72h, 7days and 2 weeks after larval treatment with seed powders. All treatments caused higher mortality in the 2nd than in the 4th instar larvae, compared with zero% mortality in the control. Larval growth inhibited, and pupation drastically reduced to 72, 60, 58%, after treatment with date pits, custard apple and mandarin, respectively, compere with 98% in the control. Moreover, this new bio-pesticide had a latent effect, until the emergence of adult moths, with many new scores of malformations. Samples of the treated larvae were electrophoretically examined and the total body proteins of the 4th instar larval, treated with these compounds, performed by SDS-PAGE. Control and different treatments were separated into 24 different bands according to their relative frequencies (Rf values), and molecular weights (MW). The strong efficacy of the treated compounds on protein gel electrophoresis led to detection of new bands, and disappearance of some bands in comparison to control sample. Therefore, we recommend the use of this natural materials, as dust application, for the control of insect pests on their hosts under agricultural fields conditions.