ARCHIVES
VOL. 6, ISSUE 4 (2021)
Estimation of uncertainty and variability for sample processing in brinjal (Eggplant) for pesticide residue analysis using organophosphate pesticide 14c-chlorpyrifos
Authors
Manish Kumar Sachdeva, Dileep K Singh
Abstract
Determination of true values for pesticide residues in food commodities ensures the credibility of the protocol followed in an analytical laboratory. Various proposed protocols for residue analysis fail to monitor the MRLs fixed by governmental regulatory agencies. The central inertia behind such outcomes lies in the undefined methodology followed, where the nature of the matrix, its way of processing, storage, and analytical approach is never well-thought-off. In the present study, for the presence of spiked 14C-labeled organophosphate pesticide Chlorpyrifos the pretreated brinjal extracts were analyzed on Beckman’s liquid scintillation counter. This highly sensitive technique to detect minuscule loss of pesticide during sample processing is preferred and recommended for many residue analysis laboratories. The extract obtained after sample processing steps viz. chopping, fine blending, etc., contained the analyte and pigment. These co-extracts would not affect the analysis, as an additional cleanup step removed them. The propensity of pesticide residues loss increments as the number of steps increases, but the additional cleanup step to remove co-extracts did not influence the results. In the current context, diminutive loss of radioactivity at any step is directly proportional to pesticide loss; therefore, after the conclusion of each step, the radioactivity left in the glassware or container used was thoroughly measured and were taken into account for calculating the final residue levels. Homogeneity, statistically denoted as sampling constant (KS value), of the analyte is another very significant factor that entails the certainty of pesticides residues protocol. The sampling constants and combined uncertainty with the Brinjal matrix carried almost similar values, whereas the percentage recovery and reproducibility values calculated showed remarkable distinctions in two processing conditions.
Download
Pages:36-41
How to cite this article:
Manish Kumar Sachdeva, Dileep K Singh "Estimation of uncertainty and variability for sample processing in brinjal (Eggplant) for pesticide residue analysis using organophosphate pesticide 14c-chlorpyrifos ". International Journal of Entomology Research, Vol 6, Issue 4, 2021, Pages 36-41
Download Author Certificate
Please enter the email address corresponding to this article submission to download your certificate.