ARCHIVES
VOL. 6, ISSUE 4 (2021)
Method validation using 14c-chlorpyrifos as an internal standard analyte to determine the uncertainty and variability in multi-step processing of potato during pesticide residue analysis
Authors
Manish Kumar Sachdeva, Dileep K Singh
Abstract
Residue analysis of pesticides in the food matrices often includes multi-step and mass transfer protocols. According to the nature of the food matrix, these protocols are standardized. During the sample processing steps, mass transfer of food matrix is the primary source of not obtaining the precise results. The use of radioactivity for minimal loss of pesticide residues during sample processing makes the approach robust and sensitive enough to determine the sources of uncertainty. Factors that make the results uncertain lead to variability in the analysis outcomes from one analytical laboratory to another. In the present study, 14C-chlorpyrifos was used to determine the amount of loss of the radioactivity at each step, indicating the amount of pesticide residue loss when following such protocols involving mass transfer procedure. After each transfer step, to recover the lost pesticide residues from the container where matrix samples were processed, the spiking solvent acetone was used. The amount of radioactivity determined on the Liquid Scintillation Counter after extraction and processing were added to the total amount of radioactivity obtained from surface run-off loss of radiolabeled pesticide and the loss due to spillage. The sampling constants (KS) is the weight of the matrix withdrawn from a homogeneously processed sample with relative sampling uncertainty. Sample processing for potatoes as the food matrix in the present study showed better KS values and hence CV COMB. SP% values under the ambient conditions compared to those processed under dry ice conditions.
Download
Pages:23-28
How to cite this article:
Manish Kumar Sachdeva, Dileep K Singh "Method validation using 14c-chlorpyrifos as an internal standard analyte to determine the uncertainty and variability in multi-step processing of potato during pesticide residue analysis ". International Journal of Entomology Research, Vol 6, Issue 4, 2021, Pages 23-28
Download Author Certificate
Please enter the email address corresponding to this article submission to download your certificate.