International Journal of Entomology Research

International Journal of Entomology Research


International Journal of Entomology Research
International Journal of Entomology Research
Vol. 6, Issue 3 (2021)

Screening and isolation of protease producing bacteria from Leiochrinus nilgirianus


Anusha UB, C Nija

Protease enzymes are ubiquitous in nature and usually found in all living organisms. The present study was conducted for screening bacteria with the ability to produce extracellular proteases. This study was conducted to screening and isolation of protease from bacteria present in the gut of Leiochrinus nilgirianus. Based on experiments, three bacterial strains produced clear transparent zone into casein agar plate were identified as protease producing bacteria. Different culture parameters such as pH and temperature were optimized for enzyme production. According to the morphological and biochemical tests, the isolated strains were identified as Enterobacter sp., Bacillus sp. and Aeromonas sp. The first strain Enterobacter sp. showed high potentiality for maximum protease production (0.29 U/ml) at pH 7.0 after 24 h of incubation at 40°C in a medium containing 1.0% casein. The scavenging action of Leiochrinus nilgirianus on sooty mould deposition on coconut palms is due to the presence of protease from Enterobacter sp. The present information of protease activity from bacteria present in the gut of Leiochrinus nilgirianus could assist the future attempts to unravel the scavenging action of insect and will provide the much needed impetus to agricultural development.
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How to cite this article:
Anusha UB, C Nija. Screening and isolation of protease producing bacteria from Leiochrinus nilgirianus. International Journal of Entomology Research, Volume 6, Issue 3, 2021, Pages 121-124
International Journal of Entomology Research International Journal of Entomology Research