DNA barcoding as a molecular diagnostic tool for the species authentification and phylogenetic analysis of Copera marginipes (Odonata: Plactycnemidae)
Jisha Krishnan EK, Sebastian CD
DNA barcoding is a new genomic technology for recognizing and ordering species. The utility of mitochondrial genes for resolving genus level phylogenetic relationships are well known. The present study investgated the utility of DNA barcoding as a molecular diagnostic tool for the species authentification and phylogenetic analysis of a damselfly species Copera marginipes using cytochrome oxidase I gene. The partial coding sequence yielded 616 bp sequence and BLAST analysis confirmed its molecular identity as the same species. Phylogenetic analysis confirmed the taxonomic identity of this species as Copera marginipes due to sister taxa relationship with the same species reported from Gujarath and Malaysia. The phylogenetic tree says that the ancestors of Copera genus were splitted at an earlier time with Copera nyansana and Copera sikassoensis were found in one clade and Copera marginipes from Kerala and Malaysia in another clade Phylogenetically its nearest member was found to be Copera nyansana and Copera sikassoensis with respective divergence of 16.02 and 17.18%.