Lantana camera belonging to the family Verbenacea, is a cosmopolitan invasive species with several medicinal uses in traditional medication system. It can be used to cure health problems such as; antiulcer, analgesic, anti-inflammatory, antimicrobial, anthelmintic, anticancer, antifungal. Therefore, the current study was aimed to evaluate the larvicidal effect of acetone extract of L. camara leaves and flowers against Anopheles arabiensis and Culex quinquefasciatus larvae. Results showed that larvicidal activities of L. camara leaves and flower acetone extracts against the 4th instars larvae of A. arabiensis and C. quinquefasciatus after 24-hour exposure at concentrations of 85.0, 74.38, 63.75, 53.13and 21.25ppm showed a range of mean mortality of 17% - 97% on A. arabiensis and 18% - 92% on C. quinquefasciatus larvae. The probit analysis showed that, the LC50’s was 34.67ppm and 32.35 ppm for A. arabiensis and C. quinquefasciatus larvae, respectively, while the LC95’s was 79.43ppm and 89.12 ppm, followed the same order of larvae. The flower acetone extract at concentrations of 99.12, 86.73, 74.34, 61.95and 49.56ppm showed a range of mean mortality of 37% - 78% on A. arabiensis and 35% - 92% on C. quinquefasciatus larvae. The probit analysis showed that, the LC50’s were 66.06ppm and 60.25 ppm for C. quinquefasciatus and A. arabiensis larvae, respectively, while the LC95’s were 104.71ppm and 102.32ppm, followed the same order of larvae. According to the obtained LC50 values, it was clear that, the acetone extract of L. camara leaves showed relatively higher larvicidal potentiality against C. quinquefasciatus larvae than A. arabiensis larvae. Also, leaves extract was more potent than the flower extract. Current results confirm that the use of Lantana camara leaves and flowers extracts in mosquito control instead of synthetic insecticides could be a better approach to reduce the control cost and environmental pollution.