The present study was carried out to make a preliminary checklist and evaluate the species abundance, seasonal distribution and diversity (richness, dominance and evenness) of moth fauna (Lepidoptera: Heterocera) in Rajshahi University Campus (RUC), Bangladesh from December 2017 to November 2018. A total number of 674 individuals of moth were found which belong to 29 species and 27 genera under 8 families. Among the species, the Tropical tiger moth, Asota caricae (n= 56) was the most dominant, with 8.31% of the total number of moth fauna, and the lowest population was Anomis flava and Pyrrharctia isabella (n= 2), and their relative abundance (RA) was 8.32 and 0.30% respectively. There were 3 very common, 6 common, 11 fairly common, 6 rare and 3 very rare species. The maximum diversity was recorded from the family Erebidae (sp. 11, 37.93%) whereas minimum diversity was from the family Arctiidae, Pyralidae, Sphingidae and Tineidae (sp. 1, 3.47%). About 60% of the total moth fauna were collected in the autumn season (Oct-Dec), while only around 7% was collected in the winter (Dec-Feb) and summer (Mar-May) season. From the monthly samples, the highest prevalence of moth was found in the month of November (n= 220, 32.64%) and lowest in the month of February (n= 4, 0.59%). Diversity indices of Shannon (H') and Simpson (1-D) were 1.39 and 0.98 respectively according to collected individuals. The Margalef (DMg) and Menhinick (DMn) richness index were 4.30 and 1.12, and the Berger-Parker dominance (d) and Pielou’s evenness index (J') were 0.08 and 0.41 respectively. Perhaps this works is the first report and provides baseline information for checklist and diversity of moths in RUC, Bangladesh.