The acaricidal potential of Centaurea aegyptiaca L. (Family Asteraceae) extracts was assessed against adult females and one day old eggs of Tetranychus urticae Koch (Acari: Tetranychidae). Of five main C. aegyptiaca extracts, miticidal activity-directed separation, pet. ether, methylene chloride and ethyl acetate fractions. Application of various chromatographic methods for the most potent fractions and spectral measurements (EI-MS, ESIMS, 1D-NMR, 2D-NMR) have been resulted in isolation and structural identification of six secondary metabolites reported from this plant species for the first time named, β-amyrin (1), methyl 8α-(3,4-dihydroxy-2-methylene-butanoyloxy)-6α, 15-dihydroxy-elema-1,3,11(13)-trien-12-oate (2), β-Sitosterol-3-O-β-D-glucopyranoside (3), Syringaresinol (4), p-Hydroxy benzoic acid (5) and methyl 8α, 6α, 15-trihydroxy-elema-1,3, 11 (13)-trien-12-oate (6). The sensitivity of adult females and one day old eggs of T. urticae to various fractions and isolated nature products revealed great variation in effectiveness in comparison with Azadirachtin (Okios 3.2% EC). Amongst the tested fractions and isolated compounds, ethyl acetate fraction, Syringaresinol (4) and methyl 8α, 6α, 15-trihydroxy-elema-1, 3, 11 (13)-trien-12-oate (6) were the most potent products using leaf-dipping technique against adult females after 3 and 7- days of exposure. While, pet. ether fraction and methyl 8α-(3,4-dihydroxy-2-methylene-butanoyloxy)-6α, 15-dihydroxy-elema-1,3,11(13)-trien-12-oate (2) exhibited the most toxic natural products to one day old eggs of T. urticae.