Silk proteins are mainly composed of fibroin and sericin and both these have wide range of applications. In the present study the silk protein was extracted from the yellow cocoons of Bombyx mori and it was characterized by FT-IR and GC-MS analysis. The IR spectra showed major peaks at 3329.14 cm-1 and at 1641.42 cm-1 and GC-MS analysis on showed the presence of amino acids such as serine, alanine, glycine and leucine. Further the silk protein was mainly evaluated for its antioxidant and antimicrobial property. The antioxidant activity was studied by the DPPH method and the IC50 was found to be 50.4 μg/ml. This showed good scavenging activity against free radicals by silk proteins. An antimicrobial study was studied and highest activity of 250 μg/ml was observed against Staphylococcus aureus MTCC 902.