Pollination is a major phenomenon in flowering plants which determines production efficiency. The present paper is a first attempt to provide collective information on the diversity of Insect fauna, engaged in foraging activity and pollination of Calotropis procera (AIT.) R. Br., in the desert of Thar. This xerophytic perennial shrub is widely distributed in the Great Indian Desert and is well known for its variety of medicinal properties. The collected 27 species of Insect pollinators were identified, which belongs to 3 orders (Hymenoptera, Diptera and Hemiptera), 11 families (Apidae, Formicidae, Scoliidae, Colletidae, Megachilidae, Halictidae, Vespidae, Dolichopodidae, Calliphoridae, Muscidae, Lygaeidae) and 14 Tribes (Anthophorini, Apini, Camponotini, Campsomerini, Hylaeinae, Anthidiini, Megachilini, Ropalidiini, Meliponini, Xylocopini, Chrysosomatini, Lucilian, Muscini, Lygaeini). The abundance of insect pollinators has been observed, significantly different in the different time intervals of the day (P<0.05) but t-test analysis [t-stat: -0.3527, P (T≤t: 0.3635)] suggesting, there is no significant difference in the abundance of insect pollinators in the summer and winter season. Simpson index of diversity (1-D) indicating high diversity in the study area with estimated Margalef’s richness index (M-summer 3.503 & M-winter 3.555) in both seasons. Value of Shannon index in both the seasons, representing every species in the sample.