The present work emphasizes on the biological characters, namely morphometric study of fruit fly Bactrocera umbrosa Fabricius that originated from North Sumatera, Indonesia. The fruit flies sampling was carried out at the fruit star crops that located in Namoriam village, Deliserdang district. Fruit flies identification was done by morphology and molecular approaches. The morphological traits that be observed and measured were body length (Y), wing length (X1), wing width (X2), hind leg length (X3) and tibia length (X4). The morphometric features measuring were done by using software Carl Zeiss Imaging System Axio Vision LE Release 4.8.2 under Stereo Zeiss-Stemi 2000-C microscope. Morphometric traits was analyzed descriptively. Meanwhile, the relationship among body length and other morphological traits be analyzed by stepwise multiple regression with the helping of software IBM SPSS Statistics 22. The research result showed, the fuit flies that be collected from star fruit crops based on both morphology and molecular (mtCOI DNA gene) approaches belong to Bactrocera umbrosa Fabricius. The length of mtCOI DNA gene of fruit flies was 688 bp. The mean of body length, wing length, wing width, hind leg length and tibia length of obtained fruit flies Bactrocera umbrosa were 7.87, 5.92, 2.78, 5.38 and 1.79 mm, respectively. Due to relationship among body length (Y) toward other morphological traits (X1-X4), the tibia length has the highest contribution in determination of body length of fruit flies through linier regression equation model Y = 5.64 + 1.24X4 (r2=36.30%).