Castor is the most important non edible oil seed crop. Castor is cultivated all over the world because of its oil and derivatives. Insect pests affecting castor are the major biotic factors in lowering of castor yield. Among that, Tobacco Caterpillar, Spodoptera litura (Fab.) (Noctuidae: Lepidoptera), is a major defoliator of castor. The management of S. litura often becomes difficult as there are no identified sources of resistance in castor against this pest. S. litura larvae are also less susceptible to insecticides. Hence, development of insect resistant castor is one of the promising approaches for management of the major Lepidopteron defoliator like S. litura. The present study was undertaken to analyse the antixenosis mechanism of resistance in selected castor accessions against Tobacco caterpillar S. litura. Based on the preliminary study, the promising accessions viz., IVHT-39, DCS-107, Salem Local, IVHT-29, IVHT-38 along with a susceptible check, IVHT-35 were selected. Feeding preference of S. litura larvae to the leaves of IVHT-39 and Salem Local was minimum in the confinement test. Salem Local recorded minimum feeding preference in free choice test. Ovipositional preference of S. litura adults, the accession Salem Local was least preferred in confinement test, whereas in free choice test IVHT-39 was least preferred.