Extracts of Zingiber officinale, Erythrophleum africanum, Calotropis procera, Nicotiana tabacum and Datura metel were assayed for lethal activity against 4^th instar larvae of Anopheles gambiae. Activity guided fractionation of the extracts with organic solvents was employed. Each fraction obtained was formulated into 4.0mg/mL concentration and tested against 25 larvae over 12 to 72 hrs. Phytochemical screening, column and thin-layer chromatography as well as GC/MS of the extract were used for searching active ingredients. Data was analyzed through Analysis of Variance and differences between means were separated with DNMRT. Aqueous extract of N. tabacum exhibited highest lethal activity (100% mortality) of the larvae. Ten column fractions were determined for the extract, with Meth III fraction causing 80% mortality. GC/MS revealed that, Bicyclo (2, 2, 1) heptane 3-methylene-2, 2,-dimethyl-5-o1 acetate (62.40%) was the major compound. The compound could be responsible for lethal activity, thus, may be useful candidate for further investigation.