Insecticides are reported to have the potency to cause neurotoxicity in non-target organisms as well. Organochlorine pesticides are widely used in agricultural practices. The present study is regarding the neurotoxic effect of Dicofol, a miticide on freshwater fish Channa punctatus. The neurotoxic effect of this insecticide was evaluated using various hematological and biochemical parameters after exposure to two different sublethal concentrations of Dicofol i.e., 35 µL and 50 µL in 15L of water at varying exposure periods of 3, 5, 10 and 15 days. Behavioural study of the fish specimen was also conducted along with analysis of various physicochemical parameters of water. During different exposure times variation in different parameters was observed. It may be inferred from the results obtained that Dicofol causes neurotoxicity in the fish, and is hence neurotoxic to other aquatic and terrestrial species.