Success of Sericulture industry mainly depends on utilization of productive breeds and high nutritious mulberry leaves. Chemical composition of mulberry differs sharply among different mulberry varieties. The present study was conducted to know the influence of two mulberry varieties V₁ and S₃₆ on aminotransferase enzymes as well as economic parameters in the silkworm breeds viz., CSR₂, CSR₄, NB₄D₂ and Diazo. The silkworm larvae reared with V₁ variety performed better in respect of larval weight, cocoon weight, shell weight, shell percentage, pupation rate, filament length, denier, renditta, reelability percentage and raw silk percentage as compared to S₃₆. Further, maximum activity of aminotransferase enzymes were registered in the silkworm larva reared on V₁ variety. However, the aspartate aminotransferase activity was more when compared to alanine aminotransferase in the silkworm batches reared with V₁ and S₃₆ varieties.