Present investigation has made an attempt to assess the physico-chemical status of water of two natural perennial fishery ponds of Balrampur district of Uttar Pradesh, India and also established the variations in the diversity of aquatic insects of the study areas. The physico-chemical parameters values obtained are in the range of international standards values as well as other scientist works. The diversity of insect fauna of these two freshwater bodies was represented by 32genera belonging to 20 families and 6 orders. Among the aquatic insects collected from both fresh water bodies, the order Hemiptera (15 genera) was dominant and followed by order Odonta (7 genera), Ephemeroptera (3 genera), Diptera (3 genera), Coleoptera (2 genera) and Trichoptera (2 genera).