The present study aims at attempting to describe morphology, morphometry, PCA BLAST analysis and phylogenetic construction of Leucauge decorata spider of suburban area of south Bangalore from Turahalli forest region. The five males and six female L.decorata spiders were collected and preserved in 70% alcohol for morphological study. Few were stored in -800C freezer for DNA extraction and for further analysis. The morphological study revealed that females were larger in size (5.60mm) compared to males (1.13mm). The PCA data revealed that 1st principle component accounts for 83.873 % of total variance (0.271 weightage) on opisthosoma breadth ventral view as its dominant term and the 2nd principle component whose dominant term was the total body breadth ventral view with a 7.891 % of total variance (weightage 0.035). The points filled as two obvious clusters confirming the succinct sexual dimorphic feature of the spiders. The DNA extraction was carried over by cTAB method. cytochrome oxidase I gene (CoxI) has a significance of exhibiting fast mutation rate hence, forward and reverse primers were constructed and PCR analysis was performed. BLAST programme was employed to find the similarity between the closely related species and maximum likelihood method was used to construct phylogenetic tree. The branch point or internal nodes represented the most common ancestor. At each branch point lies the most recent common ancestor of all the groups descended from that branch point. Finally, the BLAST analysis gave 100% similarity matching to Leucauge decorata spider from the already existing database