Present study was conducted to ascertain food preference in some species of Drosophila in natural and artificial condition as well as to determine whether wild type drosophilid grow in artificial food medium or not. Different baits were used to ascertain the food preference.Only 3 species of genus Drosophila viz., Drosophila suzukii, Drosophila immigrans and Drosophila busckii were observed from different bait. Flies collected from their natural habitat were transferred to the yeast containing prepared food medium. After 15-20 days of observations, it was found that almost all flies survived in the yeast prepared food medium and within few days, they reproduced to produce offspring with less mortality. On the contrary, when flies were transferred to the yeast free artificial food medium, it was found that all flies had died.