Diversity analysis, DNA barcoding and phylogenetic analysis of Paederinae of north Kerala were performed. Cytochrome Oxidase I gene of 18 species of Paederinae were sequenced and deposited in NCBI (all new to the database). The present analysis confirms the current placement of Paederini and Lathrobiini as distinct tribes. The two major subtribes of Paederini namely Paederina and Cryptobiina formed as sister clades and the members of genera Cephalochetes and Ochthephilum shared monophyly which is in congruence with their morphologic characters as both of them have synapomorphic geniculate antennae. The clustering of species was similar based on morphology and molecular data and hence the robustness of the tree is ensured for better prediction of phylogeny which clearly demonstrates the advantage of using both the data simultaneously for phylogeny.