The ovicidal and larvicidal effects of the tested insecticides, Chlorantraniliprole (Coragen 20% SC) Indoxacarb (Steward 15% SC), Emamectin benzoate (Basha) and Pyridalyl (Pleo 50% EC) on the 4^th larval instars and eggs of 1,2 and 3 days old of Spodoptera littoralis was studied by demonstrating total protein content, SDS-PAGE, α and β-esterase profile changes using polyacrylamide gel electrophoresis. All tested insecticides had different effects on total protein content according to the tested stages, 4^th instar larvae, and eggs age. The total protein of 4^th larval instars recorded highly significant reduction with all treated insecticides and the most reduced one was Pleo. No significant difference in total protein in the eggs of the same age and insecticides and highly significant difference between each insecticide and different egg age. The 3 days old eggs showed a non-significant reduction in all treatment, especially in the cases of Pleo and the lowest effect was Coragen treatments. While the protein of 2 days old eggs was increased with all treatment and the most increased was Coragen and Pleo in addition to the treatment with Coragen and Basha in case of one-day-old eggs. The electrophoretic pattern of proteinshowed significant effects on protein bands someof them are common among treated and untreated and others disappeared or created and specific for each insecticide in both eggs and larval samples. 40, 45, and 64 protein fractions detected in eggs of (1,2) and 3 days old with (four) and eight dominant bands, respectively. Proteins of the larvae detected 66protein bands with seven dominant bands. The α esterase bands of larvae separated into 33 bands with 3 dominant bands. While 3 days old eggs detected 27 with two dominant bands. In addition to β-esterase of larvae were 39 bands with five dominant bands, while 27 Β-esterase bands of 3 days old eggs were detected with two dominant bands.