Hyblaea puera Walker is one of the most important pests of teak from their seedling stage to plantations and mature trees in India. The virulence effect and progeny production of new to science species of entomopathogenic nematode (EPN), Steinernema dharanaii (TFRIEPN-15) was determined through different doses test in the laboratory. The counted numbers of Infective Juveniles (IJs) of S. dharanaii nine doses ranges from 3 to 100 IJs larva^-1 were exposed for infectivity and progeny production. The result exhibited dose-dependent larval mortality. The result exhibited that the larvae of H. puera are susceptible to all doses of S. dharanaii. The lowest dose of 3 IJs larva^-1 caused 34.28 percent. The 100 per cent larval mortality obtained in 40 IJs larva^-1 dose after exposed to 72 hours. The progeny production of infective juveniles from the cadavers of H. puera showed dose-dependent relationship. It was found that the lower production (7766.6) of IJs in lower dose 3 IJs larva^-1 and higher production (14,880) in 30 IJs larva^-1, but also found that higher dose 40 IJs larva^-1 the progeny production decreased (4,900). The LC₅₀, LC₉₀ and LT₅₀, LT₉₀ were also calculated. This research indicates the effectiveness of locally isolated EPNs species for controlling teak defoliator, H. purea larvae.