International Journal of Entomology Research

Authors
Abdul Mannan Hamzah, Mansoor ul Hassan, Ata ul Mohsin, Salman Ghuffar, Nasir Mehmood, Abdul Qadir, Khalid Mehmood, Muhammad Sajid Qureshi, Ehsan ul Haq, Hafiz Muhammad Asadullah, Muhammad Zeshan Ahmed

Abstract
Effectiveness of two entomopathogenic fungi, Beauveria bassiana (Balsamo) and Metarhizium anisopliae (Metschn.) against Rhyzopertha dominica was checked under in vitro condition. During the study, a homogenous culture of lesser grain borer was developed and tested under controlled lab conditions. Three concentrations (1×10⁸, 5×10⁷ and 25×10⁶ CFU) of each fungus were used by spraying 50 gram of wheat in the combined and separate form at 30±2^oC with 65±5% R.H. Pacer ^(R) (Metarhizium anisopliae) and RACER ^(TM) (Beauveria bassiana) commercially available product by Agri Life India were considered in this study. Mortality data were recorded at three exposure time intervals (5, 10 and 15 days). Research outcomes publicized that by increasing the concentration of fungi their efficacy was also increased.Moreover, in the combined application of (Beauveria bassiana+ Metarhizium anisopliae) gave the highest mortality percentage of Rhyzopertha dominica F. as compared to a single application.