Cytogenetic analyses have been carried on Disparoneura quadrimaculata, Esme cyaneovittata, Esme longistyla, Prodasineura nigra and Prodasineura verticalis of subfamily Disproneurinae by conventional staining, C-banding, silver nitrate staining and sequence specific staining. All the species possess n=13 as haploid chromosome number with X0-XX sex determining mechanism, while m chromosomes are present only in the two species of genus Prodasineura. Mostly, dark terminal C-bands are present on the autosomal bivalents of all the species, while 3 bivalents in Disparoneura quadrimaculata and 4 bivalents in Esme cyaneovittata possess light terminal C-bands. X chromosome shows terminal C-band on one side in Disparoneura quadrimaculata, while it is C-positive in Esme cyaneovittata, Esme longistyla and Prodasineura verticalis. Whereas in all the species, dark terminal NOR’s are seen on the autosomal bivalents, while 1 bivalent of Prodasineura verticalis and 3 bivalents of Disparoneura quadrimaculata show dark terminal NOR only on one side. X chromosome possesses terminal NOR’s in Disparoneura quadrimaculata and Prodasineura verticalis and it is NOR rich in both the species of genus Esme. Whole chromosome complement of Esme longistyla possesses both DAPI and CMA₃ bright signals, while it is more DAPI bright than CMA₃ in Disparoneura quadrimaculata, Esme cyaneovittata and Prodasineura verticalis.