Trichomes are the physical and chemical barrier present on the aerial surface of different plant parts which place an important role against insect herbivores. Aerial surface tissues of tomato are inhabited by diversified morphologically distinct types of trichomes which place an important role in whitefly (Bemisia tabaci) resistance. Field evaluation of 260 tomato minicore accessions during rabi and summer seasons revealed that, the trichome type played an important role in whitefly resistance on tomato. The accessions with trichome types I, II, III, IV, V and VII have recorded significantly less number of whiteflies (26.50 nymphs/ pl) whereas, significantly higher number of whiteflies were recorded from the accessions with trichome type I, III, V (41.75 nymphs/ pl). Tomato accessions having trichome type VI, IV and Type I have recorded significantly lesser number of whitefly nymphs at 45 and 60 DAT. All these trichome types are glandular trichomes with single or four lobes. Tomato accessions with high density of different glandular trichomes are correlated with the higher level of whitefly (B. tabaci) repellence.