The study reveals effect of high temperature on the total protein content level in the haemolymph, fat body and cuticle of fifth instar silkworm larvae and its effect on commercial traits among few silkworm races. 7 bivoltine and 12 multivoltine silkworm races were exposed to high temperature of 40⁰ C - 45 ⁰C and quantitative analysis of total protein profile carried out. Variation in the temperature significantly influenced the protein content in the heamolymph, fat body and cuticle of fifth instar larvae. Total protein content of heamolymph increased with increase in the temperature. The impact of heat shock on commercial traits of cocoons analyzed for acquired thermo tolerance level over the control. The data obtained was recorded for each trail in a one way ANOVA and was subjected with the Graph pad prism software analysis for its significance. Results revealed that, significantly high protein content recorded in bivoltine compared to multivoltine. Further, KNT, AP-White, GFP-C, GLPF of multivoltine strains and BO₂, BD₂S, BO1S and ISK of bivoltine strains exhibited better survivability over the other strains. Finally, all the obtained data reveals the varying response of a tolerance and non-tolerance among the silkworm strains in total protein analysis in correlation with economical traits.