During four years, an investigation was conducted to examine the virulence profiles of eight strains of Trypanosoma congolense Forestisolated from domestic animals in four sites of African Animal Trypanosomosis (TAA) in the west of the Democratic Republic of Congo. Virulence tests conducted in NMRI mice revealed that, five of those strains (62.5%) had an average virulence profile and three of them had low virulence profile (37.5%). Both strains of Kimwenza and one of N'Djili Brasseries in the city of Kinshasa Province, showed a moderate virulence profile. At Mbanza-Ngungu in Kongo Central Province, two strains revealed low virulence profile and another strain an intermediate virulence profile while at Mushie in the Province of Mayi Ndombe, a strain had a low virulence profile and another a moderate virulence profile. The low profile virulence strains showed a prepatent period between 10 ± 4.5 and 20 ± 4.9 days while those of intermediate virulence profile had a prepatent period between 2 ± 0.0 to 6 ± 3.7 days. The results of this study revealed that the disease should normally have a low impact on the health of animals and livestock.