Two species Megachile nana and Megachile saphira of subgenus Chelostomoda Michener under the genus Megachile Latreille have been reported for the first time from plains of Sutlej basin in northwestern India in the states of Punjab and Haryana. Megachile nigroluteus sp. n. has been described as a new species. The important morphological taxonomic characters has been described and illustrated. In addition floral associations and morphological measurements have also been recorded in all the three species studied presently.