Major proteases in the gut of mosquito larvae are trypsin-like serine proteases. Plants produce protease inhibitors and they play an important role in the defense of plants against pests and pathogens. In this study we screened different plant extracts to identify extracts containing inhibitors against trypsin-like gut proteases of 4th instar larvae of Aedes albopictus. Initial screening was done using bovine trypsin and the extracts showing greater than 50% trypsin inhibition was further tested against the gut proteases of Aedes albopictus larvae. From 27 plant extracts selected for initial screening, 19 plant extracts showed trypsin inhibition greater than 50%. These plant extracts were used to test the gut protease inhibition of A. albopictus larvae and 7 plant extracts showed greater than 50% inhibition. Purification and characterization of plant protease inhibitors from these plant extracts can be exploited for mosquito control.