In order to develop an environment-friendly mosquitocides an alternative to the chemical mosquitocides, extracts were made from the leaves of Rauvolfia serpentina with three organic solvents such as methanol (ME), Acetone (AN), and petroleum ether (PE). The objective of the present research is to determine the mosquitocidal activity of Rauvolfia serpentina with three different solvents against Culex quinquefasciatus. Bioassay tests were carried out by WHO guidelines for determination of mosquitocidal activities. All the solvent extracts showed promising mosquitocidal effect, however the effectivity was found highest in the metholic leaf extract. The present investigation suggests that the possible medicinal use of plant extracts as it provided an excellent potential for controlling Culex quinquefasciatus.